› 8urq

Electron Microscopy
3.3Å resolution

Spo11 core complex with gapped DNA

Released:
DOI: 10.2210/pdb8urq/pdb
PDB entry 8urq coloured by chain, front view.
PDB entry 8urq coloured by chain, side view.
PDB entry 8urq coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structures of the Spo11 core complex bound to DNA.
Yu Y, Wang J, Liu K, Zheng Z, Arter M, Claeys Bouuaert C, Pu S, Patel DJ, Keeney S
Nat Struct Mol Biol (2024)
PMID: 39304764
Related structures: EMD-42497

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-275867 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (5 distinct):
Meiotic recombination protein REC104 Chain: F
Molecule details ›
Chain: F
Length: 182 amino acids
Theoretical weight: 20.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P33323 (Residues: 1-182; Coverage: 100%)
Gene names: REC104, YHR157W
Sequence domains: Meiotic recombination protein REC104
Meiotic recombination protein REC102 Chain: B
Molecule details ›
Chain: B
Length: 264 amino acids
Theoretical weight: 30.26 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02721 (Residues: 1-264; Coverage: 100%)
Gene names: L8543.17, REC102, YLR329W
Sequence domains: REC102 protein
Meiosis-specific protein SPO11 Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 50.02 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23179 (Residues: 1-398; Coverage: 100%)
Gene names: SPO11, YHL022C
Sequence domains:
Antiviral protein SKI8 Chain: C
Molecule details ›
Chain: C
Length: 397 amino acids
Theoretical weight: 44.31 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02793 (Residues: 1-397; Coverage: 100%)
Gene names: REC103, SKI8, YGL213C
Sequence domains: WD domain, G-beta repeat
gapped DNA Chain: D
Molecule details ›
Chain: D
Length: 71 nucleotides
Theoretical weight: 21.94 KDa

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-42497
Expression system: Spodoptera frugiperda

Quick links