› 8urx

Electron Microscopy
6.6Å resolution

Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 30 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome

Released:
DOI: 10.2210/pdb8urx/pdb
PDB entry 8urx coloured by chain, front view.
PDB entry 8urx coloured by chain, side view.
PDB entry 8urx coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of RfaH-mediated transcription-translation coupling.
Molodtsov V, Wang C, Zhang J, Kaelber JT, Blaha G, Ebright RH
Nat Struct Mol Biol (2024)
PMID: 39117885
Related structures: EMD-42503

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 67-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280378 (preferred)
Entry contents:
58 distinct polypeptide molecules
2 distinct DNA molecules
5 distinct RNA molecules
Macromolecules (65 distinct):
Large ribosomal subunit protein bL21 Chain: 0
Molecule details ›
Chain: 0
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG48 (Residues: 1-103; Coverage: 100%)
Gene names: JW3153, b3186, rplU
Sequence domains: Ribosomal prokaryotic L21 protein
Large ribosomal subunit protein uL22 Chain: 1
Molecule details ›
Chain: 1
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P61175 (Residues: 1-110; Coverage: 100%)
Gene names: JW3277, b3315, eryB, rplV
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein uL23 Chain: 2
Molecule details ›
Chain: 2
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADZ0 (Residues: 1-100; Coverage: 100%)
Gene names: JW3280, b3318, rplW
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL24 Chain: 3
Molecule details ›
Chain: 3
Length: 104 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60624 (Residues: 1-104; Coverage: 100%)
Gene names: JW3271, b3309, rplX
Sequence domains:
Large ribosomal subunit protein bL25 Chain: 4
Molecule details ›
Chain: 4
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P68919 (Residues: 1-94; Coverage: 100%)
Gene names: JW2173, b2185, rplY
Sequence domains: Ribosomal L25p family
Large ribosomal subunit protein uL10 Chain: 9
Molecule details ›
Chain: 9
Length: 165 amino acids
Theoretical weight: 17.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7J3 (Residues: 1-165; Coverage: 100%)
Gene names: JW3948, b3985, rplJ
Sequence domains: Ribosomal protein L10
DNA-directed RNA polymerase subunit beta Chain: AA
Molecule details ›
Chain: AA
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
Transcription antitermination protein RfaH Chain: AB
Molecule details ›
Chain: AB
Length: 162 amino acids
Theoretical weight: 18.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFW0 (Residues: 1-162; Coverage: 100%)
Gene names: JW3818, b3842, hlyT, rfaH, sfrB
Sequence domains: Transcription termination factor nusG
DNA-directed RNA polymerase subunit alpha Chains: AC, AD
Molecule details ›
Chains: AC, AD
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: AE
Molecule details ›
Chain: AE
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: AF
Molecule details ›
Chain: AF
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
Small ribosomal subunit protein bS18 Chain: C
Molecule details ›
Chain: C
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 1-75; Coverage: 100%)
Gene names: JW4160, b4202, rpsR
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein bS20 Chain: E
Molecule details ›
Chain: E
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 1-87; Coverage: 100%)
Gene names: JW0022, b0023, rpsT
Sequence domains: Ribosomal protein S20
Small ribosomal subunit protein bS21 Chain: F
Molecule details ›
Chain: F
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 1-71; Coverage: 100%)
Gene names: JW3037, b3065, rpsU
Sequence domains: Ribosomal protein S21
Small ribosomal subunit protein uS2 Chain: G
Molecule details ›
Chain: G
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 1-241; Coverage: 100%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein bS1 Chain: H
Molecule details ›
Chain: H
Length: 557 amino acids
Theoretical weight: 61.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG67 (Residues: 1-557; Coverage: 100%)
Gene names: JW0894, b0911, rpsA, ssyF
Sequence domains: S1 RNA binding domain
Small ribosomal subunit protein uS3 Chain: I
Molecule details ›
Chain: I
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 1-233; Coverage: 100%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: J
Molecule details ›
Chain: J
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 1-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: K
Molecule details ›
Chain: K
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 1-167; Coverage: 100%)
Gene names: JW3265, b3303, rpsE, spc
Sequence domains:
Small ribosomal subunit protein bS6 Chain: L
Molecule details ›
Chain: L
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-135; Coverage: 100%)
Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: M
Molecule details ›
Chain: M
Length: 179 amino acids
Theoretical weight: 20.06 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02359 (Residues: 1-179; Coverage: 100%)
Gene names: JW3303, b3341, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: N
Molecule details ›
Chain: N
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 1-130; Coverage: 100%)
Gene names: JW3268, b3306, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: O
Molecule details ›
Chain: O
Length: 130 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7X3 (Residues: 1-130; Coverage: 100%)
Gene names: JW3199, b3230, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: P
Molecule details ›
Chain: P
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7R5 (Residues: 1-103; Coverage: 100%)
Gene names: JW3283, b3321, nusE, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: Q
Molecule details ›
Chain: Q
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 1-129; Coverage: 100%)
Gene names: JW3259, b3297, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: R
Molecule details ›
Chain: R
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS14 Chain: S
Molecule details ›
Chain: S
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 1-101; Coverage: 100%)
Gene names: JW3269, b3307, rpsN
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 Chain: T
Molecule details ›
Chain: T
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: U
Molecule details ›
Chain: U
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: V
Molecule details ›
Chain: V
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 1-84; Coverage: 100%)
Gene names: JW3273, b3311, neaA, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein uS19 Chain: W
Molecule details ›
Chain: W
Length: 92 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7U3 (Residues: 1-92; Coverage: 100%)
Gene names: JW3278, b3316, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein uS13 Chain: X
Molecule details ›
Chain: X
Length: 118 amino acids
Theoretical weight: 13.13 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7S9 (Residues: 1-118; Coverage: 100%)
Gene names: JW3260, b3298, rpsM
Sequence domains: Ribosomal protein S13/S18
Large ribosomal subunit protein uL11 Chain: Y
Molecule details ›
Chain: Y
Length: 142 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7J7 (Residues: 1-142; Coverage: 100%)
Gene names: JW3946, b3983, relC, rplK
Sequence domains:
Large ribosomal subunit protein bL12 Chain: Z
Molecule details ›
Chain: Z
Length: 121 amino acids
Theoretical weight: 12.31 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7K2 (Residues: 1-121; Coverage: 100%)
Gene names: JW3949, b3986, rplL
Sequence domains:
Large ribosomal subunit protein bL27 Chain: b
Molecule details ›
Chain: b
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7L8 (Residues: 1-85; Coverage: 100%)
Gene names: JW3152, b3185, rpmA
Sequence domains: Ribosomal L27 protein
Large ribosomal subunit protein bL28 Chain: c
Molecule details ›
Chain: c
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1X3JA73 (Residues: 1-78; Coverage: 100%)
Gene names: ECVG_03476, rpmB
Sequence domains: Ribosomal L28 family
50S ribosomal protein L29 Chain: e
Molecule details ›
Chain: e
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L30 Chain: f
Molecule details ›
Chain: f
Length: 59 amino acids
Theoretical weight: 6.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL31 Chain: g
Molecule details ›
Chain: g
Length: 70 amino acids
Theoretical weight: 7.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7M9 (Residues: 1-70; Coverage: 100%)
Gene names: JW3907, b3936, rpmE
Sequence domains: Ribosomal protein L31
Large ribosomal subunit protein uL2 Chain: h
Molecule details ›
Chain: h
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Sequence domains:
50S ribosomal protein L32 Chain: i
Molecule details ›
Chain: i
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL3 Chain: j
Molecule details ›
Chain: j
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
50S ribosomal protein L33 Chain: k
Molecule details ›
Chain: k
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL4 Chain: l
Molecule details ›
Chain: l
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A1AGK6 (Residues: 1-201; Coverage: 100%)
Gene names: APECO1_3132, Ecok1_33020, rplD
Sequence domains: Ribosomal protein L4/L1 family
Large ribosomal subunit protein bL34 Chain: m
Molecule details ›
Chain: m
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7MGC4 (Residues: 1-46; Coverage: 100%)
Gene names: ECS88_4126, rpmH
Sequence domains: Ribosomal protein L34
50S ribosomal protein L5 Chain: n
Molecule details ›
Chain: n
Length: 179 amino acids
Theoretical weight: 20.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL35 Chain: o
Molecule details ›
Chain: o
Length: 65 amino acids
Theoretical weight: 7.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Q1 (Residues: 1-65; Coverage: 100%)
Gene names: JW1707, b1717, rpmI
Sequence domains: Ribosomal protein L35
Large ribosomal subunit protein uL6 Chain: p
Molecule details ›
Chain: p
Length: 177 amino acids
Theoretical weight: 18.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: C3SR17 (Residues: 1-177; Coverage: 100%)
Gene names: A2J79_004074, A5U30_004539, ABE91_009085, ACU57_21025, AWP47_12710, B6R15_004082, B6R31_004981, BANRA_01936, BANRA_04315, BCB93_004280, BG944_001120, BGM66_003606, BGZ_04112, BK300_26865, BK383_24710, BKL28_004556, BMT50_12280, BRV02_004610, BTB68_004369, BTQ06_10625, BXT93_05540, BvCmsKKP061_03017, C0P57_002655, C1Q91_004917, C2M16_25470, C2R31_004620, C3F40_19785, C6669_11430, C719_004900, C7B02_26515, C9160_24335, C9194_22610, C9Z68_22570, CCS08_00150, CF22_004862, CG704_20935, CIG67_10740, CQ842_14390, CQ842_22280, CR538_02485, CTR35_003617, CWS33_24875, D3G36_23115, D4N09_20190, D9D43_22615, D9E49_05175, D9J61_16670, DD762_23565, DIV22_15240, DL968_17150, DNQ45_03775, DNX30_25460, DS732_24280, DTL43_20980, DU321_12020, E2863_04791, E4K51_21510, E5H86_24505, E6D34_21740, EAI46_07495, ECs4170, EIZ93_12450, EN85_004362, EPS76_07195, EPS97_20075, EWK56_25015, EXX87_25575, ExPECSC038_03653, F7F11_22705, F7N46_23990, F9413_21215, F9461_25635, F9B07_24665, FGAF848_25980, FIJ20_21175, FJQ40_18325, FKO60_25655, FOI11_019240, FOI11_03940, FPI65_20325, FPS11_25410, FTV93_20880, FVB16_04890, FWK02_06290, FZU14_21840, G3V95_19865, G3W53_20975, G4A38_21530, G4A47_20800, GAI89_24810, GAJ12_24700, GKF66_21565, GNW61_16650, GNZ05_25965, GOP25_22725, GP711_23325, GP944_18810, GP954_00925, GP965_04315, GP975_01205, GP979_01985, GQA06_03930, GQE64_20440, GQE86_20100, GQM04_10585, GQM13_25220, GQM21_11250, GQN24_22695, GRC73_21730, GRW05_09080, GRW24_04735, GTP92_23175, GUC01_21200, HEP30_018655, HEP34_004767, HHH44_004532, HI055_004143, HIE29_005190, HJQ60_005008, HKA49_005032, HLX92_10055, HLZ39_22195, HLZ50_22160, HMV95_19625, HV109_02170, HV209_14695, HVV39_09285, HVW43_18650, HVY77_02155, I6H02_12010, IFC14_004403, IH772_23995, J0541_004400, J5U05_004084, J8F57_004250, JNA68_24810, JNP96_25325, NCTC10082_02836, NCTC10279_02095, NCTC10418_00687, NCTC10764_03860, NCTC10767_01513, NCTC11112_01039, NCTC11126_02768, NCTC11181_02735, NCTC11341_01892, NCTC13148_03444, NCTC7927_00548, NCTC7928_02519, NCTC8009_01508, NCTC8333_00486, NCTC8603_03279, NCTC8621_00505, NCTC8960_03067, NCTC8985_04979, NCTC9001_04251, NCTC9044_01360, NCTC9077_00601, NCTC9081_00914, NCTC9117_00753, NCTC9702_00534, NCTC9706_02734, NOI85_25600, NY836_17905, OFN31_22800, OGM49_22350, P6223_003986, PWL68_004852, PWL68_005333, Q2V20_09685, Q2V64_25400, QDW62_02175, QO046_13775, R8G00_13260, R8O40_004924, RZR61_12910, SAMEA3472044_01159, SAMEA3752557_01955, V9Z47_02395, WR15_19625, rplF
Sequence domains: Ribosomal protein L6
Large ribosomal subunit protein bL36 Chain: q
Molecule details ›
Chain: q
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0E2L017 (Residues: 1-38; Coverage: 100%)
Gene names: G925_03522, rpmJ
Sequence domains: Ribosomal protein L36
50S ribosomal protein L9 Chain: r
Molecule details ›
Chain: r
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L13 Chain: s
Molecule details ›
Chain: s
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL14 Chain: t
Molecule details ›
Chain: t
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: S1NW45 (Residues: 1-123; Coverage: 100%)
Gene names: A13A_03633, rplN
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL15 Chain: u
Molecule details ›
Chain: u
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A037Y8L6 (Residues: 1-144; Coverage: 100%)
Gene names: ACU57_21045, BANRA_01932, BGM66_003610, C0P57_002651, CIG67_10760, CTR35_003613, DL968_17170, DTL43_21000, E4K51_21490, EIZ93_12470, FOI11_019260, FOI11_03920, FWK02_06270, G3V95_19845, G4A38_21510, G4A47_20780, GNW61_16630, GOP25_22705, GP944_18790, GP954_00945, GP965_04295, GP979_02005, GQE64_20420, GRW05_09060, HMV95_19605, HVY77_02175, IH772_24015, J0541_004396, JNP96_25305, NCTC11126_02772, NCTC9045_00567, NCTC9117_00757, NCTC9706_02738, P6223_003982, QDW62_02195, RZR61_12890, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL16 Chain: v
Molecule details ›
Chain: v
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADY7 (Residues: 1-136; Coverage: 100%)
Gene names: JW3275, b3313, rplP
Sequence domains: Ribosomal protein L16p/L10e
50S ribosomal protein L17 Chain: w
Molecule details ›
Chain: w
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L18 Chain: x
Molecule details ›
Chain: x
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
50S ribosomal protein L19 Chain: y
Molecule details ›
Chain: y
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein bL20 Chain: z
Molecule details ›
Chain: z
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7MAS6 (Residues: 1-118; Coverage: 100%)
Gene names: ECS88_1766, rplT
Sequence domains: Ribosomal protein L20
NT DNA ops Chain: 5
Molecule details ›
Chain: 5
Length: 38 nucleotides
Theoretical weight: 11.79 KDa
T DNA ops Chain: 6
Molecule details ›
Chain: 6
Length: 38 nucleotides
Theoretical weight: 11.53 KDa
mRNA with 30 nt long spacer Chain: 7
Molecule details ›
Chain: 7
Length: 47 nucleotides
Theoretical weight: 14.75 KDa
E-site and P-site fMet-tRNA Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 nucleotides
Theoretical weight: 24.5 KDa
Sequence domains: tRNA
16S ribosomal RNA Chain: D
Molecule details ›
Chain: D
Length: 1542 nucleotides
Theoretical weight: 499.69 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
23S ribosomal RNA Chain: a
Molecule details ›
Chain: a
Length: 2904 nucleotides
Theoretical weight: 941.64 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
5S ribosomal RNA Chain: d
Molecule details ›
Chain: d
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.6Å
Relevant EMDB volumes: EMD-42503
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structural basis of archaeal FttA-dependent transcription termination.
You et al. (2024)