› 8utn

Electron Microscopy
3.1Å resolution

KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule (class T23L1)

Released:
DOI: 10.2210/pdb8utn/pdb
PDB entry 8utn coloured by chain, front view.
PDB entry 8utn coloured by chain, side view.
PDB entry 8utn coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM unveils kinesin KIF1A's processivity mechanism and the impact of its pathogenic variant P305L.
Benoit MPMH, Rao L, Asenjo AB, Gennerich A, Sosa H
OpenAccess logo Nat Commun 15 5530 (2024)
PMID: 38956021
Related structures: EMD-42543

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-269064 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kinesin-like protein KIF1A Chains: K, N
Molecule details ›
Chains: K, N
Length: 438 amino acids
Theoretical weight: 49.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12756 (Residues: 1-393; Coverage: 23%)
  • Best match: Q12756-2 (Residues: 1-393)
Gene names: ATSV, C2orf20, KIF1A
Sequence domains: Kinesin motor domain
Tubulin alpha-1B chain Chains: A, E, S
Molecule details ›
Chains: A, E, S
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chains: B, I
Molecule details ›
Chains: B, I
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AGU7 (Residues: 1-445; Coverage: 100%)
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand MG 4 x MG
Ligand ANP 2 x ANP
Ligand GTP 3 x GTP
Ligand GDP 2 x GDP
Ligand TA1 2 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-42543
Expression system: Escherichia coli

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