› 8utv

Electron Microscopy
3Å resolution

KIF1A[1-393] P305L mutant ADP bound in complex with a microtubule

Released:
DOI: 10.2210/pdb8utv/pdb
PDB entry 8utv coloured by chain, front view.
PDB entry 8utv coloured by chain, side view.
PDB entry 8utv coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM unveils kinesin KIF1A's processivity mechanism and the impact of its pathogenic variant P305L.
Benoit MPMH, Rao L, Asenjo AB, Gennerich A, Sosa H
OpenAccess logo Nat Commun 15 5530 (2024)
PMID: 38956021
Related structures: EMD-42551

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-245605 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AGU7 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Kinesin-like protein KIF1A Chain: K
Molecule details ›
Chain: K
Length: 438 amino acids
Theoretical weight: 49.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12756 (Residues: 1-393; Coverage: 23%)
  • Best match: Q12756-2 (Residues: 1-393)
Gene names: ATSV, C2orf20, KIF1A
Sequence domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-42551
Expression system: Escherichia coli

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