› 8uw3

Electron Microscopy
3.2Å resolution

Human LINE-1 retrotransposon ORF2 protein engaged with template RNA in elongation state

Released:
DOI: 10.2210/pdb8uw3/pdb
PDB entry 8uw3 coloured by chain, front view.
PDB entry 8uw3 coloured by chain, side view.
PDB entry 8uw3 coloured by chain, top view.
Source organisms:
Primary publication:
Template and target site recognition by human LINE-1 in retrotransposition.
Thawani A, Ariza AJF, Nogales E, Collins K
Nature (2023)
PMID: 38096901
Related structures: EMD-42637

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236879 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
LINE-1 retrotransposable element ORF2 protein Chain: A
Molecule details ›
Chain: A
Length: 1275 amino acids
Theoretical weight: 149.3 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O00370 (Residues: 1-1275; Coverage: 100%)
Sequence domains:
Complementary DNA Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: synthetic construct
Expression system: Not provided
Template RNA Chain: B
Molecule details ›
Chain: B
Length: 74 nucleotides
Theoretical weight: 23.96 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TTP 1 x TTP
1 modified residue:
Ligand DDG 1 x DDG

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-42637
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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