› 8ux5

X-ray diffraction
3.06Å resolution

OvsA M401Y/Q430N/A431F from Halomonas utahensis, a hercynine-binding variant with selenoneine-biosynthetic activity

Released:
DOI: 10.2210/pdb8ux5/pdb
PDB entry 8ux5 coloured by chain, front view.
PDB entry 8ux5 coloured by chain, side view.
PDB entry 8ux5 coloured by chain, top view.
Source organism: Vreelandella utahensis
Primary publication:
Discovery of the selenium-containing antioxidant ovoselenol derived from convergent evolution.
Kayrouz CM, Ireland KA, Ying VY, Davis KM, Seyedsayamdost MR
Nat Chem (2024)
PMID: 39143299

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ergothioneine biosynthesis protein EgtB Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 54.99 KDa
Source organism: Vreelandella utahensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A9X4Y9G9 (Residues: 1-448; Coverage: 100%)
Gene names: GLW01_00405, egtB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FE 2 x FE
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE 7B2
Spacegroup: P65
Unit cell:
a: 162.174Å b: 162.174Å c: 124.663Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.253
Expression system: Escherichia coli BL21(DE3)

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