› 8uz1

Electron Microscopy
3.6Å resolution

Straight actin filament from Arp2/3 branch junction sample (ADP-BeFx)

Released:
DOI: 10.2210/pdb8uz1/pdb
PDB entry 8uz1 coloured by chain, front view.
PDB entry 8uz1 coloured by chain, side view.
PDB entry 8uz1 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 complex.
Chavali SS, Chou SZ, Cao W, Pollard TD, De La Cruz EM, Sindelar CV
Nat Commun 15 2059 (2024)
PMID: 38448439
Related structures: EMD-42830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: J, K, L, M, N, O, P, Q, R
Molecule details ›
Chains: J, K, L, M, N, O, P, Q, R
Length: 372 amino acids
Theoretical weight: 41.52 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68139 (Residues: 6-377; Coverage: 99%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

3 bound ligands:
Ligand ADP 9 x ADP
Ligand MG 9 x MG
Ligand BEF 9 x BEF
1 modified residue:
Ligand HIC 9 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-42830

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