› 8uzy

Electron Microscopy
5.3Å resolution

The structure of the native cardiac thin filament troponin core in Ca2+-bound partially activated state from the upper strand

Released:
DOI: 10.2210/pdb8uzy/pdb
PDB entry 8uzy coloured by chain, front view.
PDB entry 8uzy coloured by chain, side view.
PDB entry 8uzy coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Troponin structural dynamics in the native cardiac thin filament revealed by cryo electron microscopy.
Risi CM, Belknap B, Atherton J, Leite Coscarella I, White HD, Bryant Chase P, Pinto JR, Galkin VE
J Mol Biol 168498 (2024)
PMID: 38387550
Related structures: EMD-42847

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-245623 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Actin, alpha cardiac muscle 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 377 amino acids
Theoretical weight: 42.06 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8W4F696 (Residues: 9-385; Coverage: 98%)
Gene name: ACTC1
Sequence domains: Actin
Troponin C, slow skeletal and cardiac muscles Chain: C
Molecule details ›
Chain: C
Length: 161 amino acids
Theoretical weight: 18.43 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P63317 (Residues: 1-161; Coverage: 100%)
Gene names: TNNC, TNNC1
Sequence domains:
Troponin I, cardiac muscle Chain: D
Molecule details ›
Chain: D
Length: 211 amino acids
Theoretical weight: 24.09 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1V520 (Residues: 1-211; Coverage: 100%)
Gene name: TNNI3
Sequence domains:
Troponin T, cardiac muscle Chain: E
Molecule details ›
Chain: E
Length: 285 amino acids
Theoretical weight: 33.94 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A5G2Q8N0 (Residues: 1-285; Coverage: 100%)
Gene name: TNNT2
Sequence domains: Troponin
Tropomyosin alpha-1 chain Chains: F, G
Molecule details ›
Chains: F, G
Length: 284 amino acids
Theoretical weight: 32.76 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P42639 (Residues: 1-284; Coverage: 100%)
Gene name: TPM1
Sequence domains: Tropomyosin

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.3Å
Relevant EMDB volumes: EMD-42847

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