› 8v36

X-ray diffraction
2.24Å resolution

Crystal structure of DHPS-3-dehydrogenase, HpsN from Cupriavidus pinatubonensis in complex with NADH

Released:
DOI: 10.2210/pdb8v36/pdb
PDB entry 8v36 coloured by chain, front view.
PDB entry 8v36 coloured by chain, side view.
PDB entry 8v36 coloured by chain, top view.
Source organism: Cupriavidus pinatubonensis JMP134
Primary publication:
Structural and kinetic insights into the stereospecific oxidation of R-2,3-dihydroxypropanesulfonate by DHPS-3-dehydrogenase from Cupriavidus pinatubonensis.
Burchill L, Kaur A, Nastasovici A, Lee M, Williams SJ
Chem Sci (2024)
PMID: 39263660

Function and Biology Details

Reaction catalysed: 
(R)-2,3-dihydroxypropane-1-sulfonate + 2 NAD(+) + H(2)O = (R)-3-sulfolactate + 2 NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-559508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfopropanediol 3-dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 46.99 KDa
Source organism: Cupriavidus pinatubonensis JMP134
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46N53 (Residues: 1-436; Coverage: 100%)
Gene names: Reut_C6092, hpsN
Sequence domains: Histidinol dehydrogenase

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 77.307Å b: 87.556Å c: 134.197Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.28
Expression system: Escherichia coli BL21(DE3)

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