8v6g

Electron Microscopy
11.16Å resolution

DNA initiation complex (configuration 1) of Xenopus laevis DNA polymerase alpha-primase

Released:

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-236675 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (6 distinct):
DNA polymerase alpha catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 1127 amino acids
Theoretical weight: 129.01 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9DE46 (Residues: 335-1458; Coverage: 77%)
Gene names: pola, pola1
Sequence domains:
DNA polymerase alpha subunit B Chain: B
Molecule details ›
Chain: B
Length: 601 amino acids
Theoretical weight: 67.19 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6DCZ1 (Residues: 1-598; Coverage: 100%)
Gene names: MGC80532, pola2, pola2.L
Sequence domains:
DNA primase large subunit Chain: C
DNA primase Chain: D
DNA template Chain: E
RNA primer Chain: F

Ligands and Environments

2 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 11.16Å
Relevant EMDB volumes: EMD-42990
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli