› 8v7g

X-ray diffraction
1.52Å resolution

Human DNA polymerase eta-DNA-gemC-ended primer-dAMPNPP ternary complex with Mg2+

Released:
DOI: 10.2210/pdb8v7g/pdb
PDB entry 8v7g coloured by chain, front view.
PDB entry 8v7g coloured by chain, side view.
PDB entry 8v7g coloured by chain, top view.
Source organisms:
Entry authors: Chang C, Gao Y

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270869 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase eta Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 48.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y253 (Residues: 1-432; Coverage: 61%)
Gene names: POLH, RAD30, RAD30A, XPV
Sequence domains:
DNA (5'-D(*CP*AP*TP*TP*GP*TP*GP*AP*CP*GP*CP*T)-3') Chain: T
Molecule details ›
Chain: T
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*(U7B))-3') Chain: P
Molecule details ›
Chain: P
Length: 8 nucleotides
Theoretical weight: 2.45 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand DZ4 1 x DZ4
Ligand GOL 1 x GOL
1 modified residue:
Ligand U7B 1 x U7B

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P61
Unit cell:
a: 98.36Å b: 98.36Å c: 82.047Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.184 0.198
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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