› 8va1

Electron Microscopy
3.4Å resolution

S. aureus TarL H300N in complex with CDP-ribitol (single tetramer)

Released:
DOI: 10.2210/pdb8va1/pdb
PDB entry 8va1 coloured by chain, front view.
PDB entry 8va1 coloured by chain, side view.
PDB entry 8va1 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Cryo-EM analysis of S. aureus TarL, a polymerase in wall teichoic acid biogenesis central to virulence and antibiotic resistance.
Li FKK, Worrall LJ, Gale RT, Brown ED, Strynadka NCJ
Sci Adv 10 eadj3864 (2024)
PMID: 38416829
Related structures: EMD-43083

Function and Biology Details

Reaction catalysed: 
n CDP-ribitol + 4-O-di((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = n CMP + 4-O-(D-ribitylphospho)(n)-di((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Teichoic acid ribitol-phosphate polymerase TarL Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 583 amino acids
Theoretical weight: 68.51 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2G1B8 (Residues: 1-562; Coverage: 100%)
Gene names: SAOUHSC_00227, tarL
Sequence domains: CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase

Ligands and Environments

1 bound ligand:
Ligand V2V 4 x V2V
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-43083
Expression system: Escherichia coli BL21(DE3)

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