› 8vbs

X-ray diffraction
3.31Å resolution

E. coli cysteine desulfurase SufS bound to SufE C51A

Released:
DOI: 10.2210/pdb8vbs/pdb
PDB entry 8vbs coloured by chain, front view.
PDB entry 8vbs coloured by chain, side view.
PDB entry 8vbs coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of the SufS-SufE complex reveals interactions driving protected persulfide transfer in iron-sulfur cluster biogenesis.
Gogar RK, Chhikara N, Vo M, Gilbert NC, Dunkle JA, Frantom PA
J Biol Chem 300 107641 (2024)
PMID: 39122000

Function and Biology Details

Reactions catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281657 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine desulfurase Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 44.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77444 (Residues: 1-406; Coverage: 100%)
Gene names: JW1670, b1680, csdB, sufS, ynhB
Sequence domains: Aminotransferase class-V
Cysteine desulfuration protein SufE Chains: C, D
Molecule details ›
Chains: C, D
Length: 138 amino acids
Theoretical weight: 15.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P76194 (Residues: 1-138; Coverage: 100%)
Gene names: JW1669, b1679, sufE, ynhA
Sequence domains: Fe-S metabolism associated domain

Ligands and Environments

No bound ligands
2 modified residues:
Ligand LLP 2 x LLP
Ligand CSS 2 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21212
Unit cell:
a: 91.492Å b: 130.567Å c: 101.185Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.231 0.272
Expression system: Escherichia coli

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