› 8vdw

X-ray diffraction
1.81Å resolution

X-Ray Crystal Structure of the biotin synthase from V. parvula

Released:
DOI: 10.2210/pdb8vdw/pdb
PDB entry 8vdw coloured by chain, front view.
PDB entry 8vdw coloured by chain, side view.
PDB entry 8vdw coloured by chain, top view.
Source organism: Veillonella parvula
Primary publication:
Discovery of a Biotin Synthase That Utilizes an Auxiliary 4Fe-5S Cluster for Sulfur Insertion.
Lachowicz JC, Lennox-Hvenekilde D, Myling-Petersen N, Salomonsen B, Verkleij G, Acevedo-Rocha CG, Caddell B, Gronenberg LS, Almo SC, Sommer MOA, Genee HJ, Grove TL
J Am Chem Soc 146 1860-1873 (2024)
PMID: 38215281

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-247758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biotin synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 40.49 KDa
Source organism: Veillonella parvula
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

5 bound ligands:
Ligand Q46 2 x Q46
Ligand SAM 2 x SAM
Ligand SF4 2 x SF4
Ligand DTB 2 x DTB
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P212121
Unit cell:
a: 47.465Å b: 70.218Å c: 195.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.227
Expression system: Escherichia coli BL21(DE3)

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