› 8ve8

Electron Microscopy
2.8Å resolution

Lineage IV Lassa virus glycoprotein (Josiah) in complex with rabbit polyclonal antibody (GP1-A epitope)

Released:
DOI: 10.2210/pdb8ve8/pdb
PDB entry 8ve8 coloured by chain, front view.
PDB entry 8ve8 coloured by chain, side view.
PDB entry 8ve8 coloured by chain, top view.
Source organisms:
Primary publication:
Defining bottlenecks and opportunities for Lassa virus neutralization by structural profiling of vaccine-induced polyclonal antibody responses.
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Müller-Kräuter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep 43 114708 (2024)
PMID: 39243373
Related structures: EMD-43168

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-272431 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (9 distinct):
Glycoprotein G1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 259 amino acids
Theoretical weight: 29.01 KDa
Source organism: Lassa virus Josiah
Expression system: Homo sapiens
UniProt:
  • Canonical: P08669 (Residues: 1-259; Coverage: 53%)
Gene names: GP-C, GPC
Sequence domains: Arenavirus glycoprotein
Glycoprotein G2 Chains: a, b, c
Molecule details ›
Chains: a, b, c
Length: 406 amino acids
Theoretical weight: 44.77 KDa
Source organism: Lassa virus Josiah
Expression system: Homo sapiens
UniProt:
  • Canonical: P08669 (Residues: 260-424; Coverage: 34%)
Gene names: GP-C, GPC
Sequence domains: Arenavirus glycoprotein
Rabbit polyclonal Fv light chain Chain: L
Molecule details ›
Chain: L
Length: 106 amino acids
Theoretical weight: 9.04 KDa
Source organism: Oryctolagus cuniculus
Rabbit polyclonal Fv heavy chain Chain: H
Molecule details ›
Chain: H
Length: 119 amino acids
Theoretical weight: 10.15 KDa
Source organism: Oryctolagus cuniculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 13 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-43168
Expression system: Homo sapiens

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