› 8vev

X-ray diffraction
3.06Å resolution

Structure of a mouse IgG antibody antigen-binding fragment (Fab) targeting N6-methyladenosine (m6A), an RNA modification, m6A nucleoside ligand

Released:
DOI: 10.2210/pdb8vev/pdb
PDB entry 8vev coloured by chain, front view.
PDB entry 8vev coloured by chain, side view.
PDB entry 8vev coloured by chain, top view.
Source organism: Mus musculus
Entry author: Aoki ST

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239752 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N6-methyladenosine (m6A) binding IgG Fab, heavy chain Chains: A, C, H
Molecule details ›
Chains: A, C, H
Length: 221 amino acids
Theoretical weight: 24.02 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
N6-methyladenosine (m6A) binding IgG Fab, light chain Chains: B, D, L
Molecule details ›
Chains: B, D, L
Length: 215 amino acids
Theoretical weight: 23.04 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

4 bound ligands:
Ligand PEG 1 x PEG
Ligand SO4 12 x SO4
Ligand 6MD 2 x 6MD
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 83.64Å b: 128.377Å c: 150.476Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.253
Expression system: Homo sapiens

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