8vgj

Electron Microscopy
2.5Å resolution

CryoEM structure of tryptase in complex with engineered conformationally rigid anti-tryptase Fab E104.v1.4DS

Released:

Function and Biology Details

Reaction catalysed:
Preferential cleavage: Arg-|-, Lys-|-, but with more restricted specificity than trypsin.
Biochemical function:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-280627 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tryptase alpha/beta-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 260 amino acids
Theoretical weight: 29.21 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q15661 (Residues: 31-275; Coverage: 95%)
Gene names: TPS1, TPS2, TPSAB1, TPSB1
Sequence domains: Trypsin
Fab E104.v1.4DS light chain Chains: E, G, I, L
Molecule details ›
Chains: E, G, I, L
Length: 217 amino acids
Theoretical weight: 23.6 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Fab E104.v1.4DS heavy chain Chains: F, H, J, K

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.5Å
Relevant EMDB volumes: EMD-43202
Expression systems:
  • Trichoplusia ni
  • Cricetulus griseus