› 8vhf

Electron Microscopy
3.51Å resolution

Cryo-EM structure of GPR119-Gs-Nb35 complex with small molecule agonist MBX-2982

Released:
DOI: 10.2210/pdb8vhf/pdb
PDB entry 8vhf coloured by chain, front view.
PDB entry 8vhf coloured by chain, side view.
PDB entry 8vhf coloured by chain, top view.
Source organisms:
Entry authors: Kim D-G, Cherezov V
Related structures: EMD-43236

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-532626 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 40.04 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Glucose-dependent insulinotropic receptor; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 615 amino acids
Theoretical weight: 67.78 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
  • Canonical: Q8TDV5 (Residues: 1-335; Coverage: 100%)
Gene names: GPR119, cybC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 8VP 1 x 8VP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.51Å
Relevant EMDB volumes: EMD-43236
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

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