› 8vjz

X-ray diffraction
1.9Å resolution

HLA-A*03:01 with WT KRAS-10mer

Released:
DOI: 10.2210/pdb8vjz/pdb
Model geometry
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PDB entry 8vjz coloured by chain, front view.
PDB entry 8vjz coloured by chain, side view.
PDB entry 8vjz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification and structural characterization of a mutant KRAS-G12V specific TCR restricted by HLA-A3.
Sim MJW, Hanada KI, Stotz Z, Yu Z, Lu J, Brennan P, Quastel M, Gillespie GM, Long EO, Yang JC, Sun PD
Eur J Immunol e2451079 (2024)
PMID: 39030753

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-272007 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-301; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
GTPase KRas, N-terminally processed Chain: C

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P622
Unit cell:
a: 154.637Å b: 154.637Å c: 85.365Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.214
Expression systems:
  • Escherichia coli
  • synthetic construct

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