› 8vle

Electron Microscopy
3.3Å resolution

Composite structure of human FASN with NADPH in State 1

Released:
DOI: 10.2210/pdb8vle/pdb
PDB entry 8vle coloured by chain, front view.
PDB entry 8vle coloured by chain, side view.
PDB entry 8vle coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Snapshots of acyl carrier protein shuttling in human fatty acid synthase.
Schultz K, Costa-Pinheiro P, Gardner L, Pinheiro LV, Ramirez-Solis J, Gardner SM, Wellen KE, Marmorstein R
Nature (2025)
PMID: 39979457
Related structures: EMD-43337

Function and Biology Details

Reactions catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+)
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 2553 amino acids
Theoretical weight: 278.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P49327 (Residues: 2-2511; Coverage: 100%)
Gene names: FAS, FASN
Sequence domains:

Ligands and Environments


Cofactor: Ligand NDP 4 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.3Å
Relevant EMDB volumes: EMD-43337
Expression system: Spodoptera frugiperda

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