› 8vlg

Electron Microscopy
3.15Å resolution

Cryo-EM structure of human HGSNAT bound with Acetyl-CoA and substrate analog

Released:
DOI: 10.2210/pdb8vlg/pdb
PDB entry 8vlg coloured by chain, front view.
PDB entry 8vlg coloured by chain, side view.
PDB entry 8vlg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and mechanistic insights into a lysosomal membrane enzyme HGSNAT involved in Sanfilippo syndrome.
Zhao B, Cao Z, Zheng Y, Nguyen P, Bowen A, Edwards RH, Stroud RM, Zhou Y, Van Lookeren Campagne M, Li F
Nat Commun 15 5388 (2024)
PMID: 38918376
Related structures: EMD-43338

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + heparan sulfate alpha-D-glucosaminide = CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Heparan-alpha-glucosaminide N-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 663 amino acids
Theoretical weight: 73.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q68CP4 (Residues: 1-663; Coverage: 100%)
Gene names: HGSNAT, TMEM76
Sequence domains: Heparan-alpha-glucosaminide N-acetyltransferase, catalytic

Ligands and Environments


Cofactor: Ligand ACO 2 x ACO
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 4 x NAG
Ligand A1ACY 2 x A1ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.15Å
Relevant EMDB volumes: EMD-43338
Expression system: Homo sapiens

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