› 8vmf

X-ray diffraction
2.5Å resolution

Crystal structure of a transition-state mimic of the GSK-3/Axin complex bound to a beta-catenin S45D peptide

Released:
DOI: 10.2210/pdb8vmf/pdb
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PDB entry 8vmf coloured by chain, front view.
PDB entry 8vmf coloured by chain, side view.
PDB entry 8vmf coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional effects of phosphopriming and scaffolding in the kinase GSK-3β.
Enos MD, Gavagan M, Jameson N, Zalatan JG, Weis WI
Sci Signal 17 eado0881 (2024)
PMID: 39226374

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-275941 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glycogen synthase kinase-3 beta Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 41.54 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WV60 (Residues: 26-383; Coverage: 85%)
Gene name: Gsk3b
Sequence domains: Protein kinase domain
Axin-1 Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15169 (Residues: 383-402; Coverage: 2%)
Gene names: AXIN, AXIN1
Catenin beta-1 Chain: C

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand AF3 1 x AF3
Ligand ADP 1 x ADP
Ligand GOL 3 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P6122
Unit cell:
a: 82.251Å b: 82.251Å c: 280.354Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.251
Expression system: Escherichia coli BL21(DE3)

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