8vmi

Electron Microscopy
3.1Å resolution

PRC2_AJ119-450 bound to H3K4me3

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-284534 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Polycomb protein EED Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 50.27 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O75530 (Residues: 1-441; Coverage: 100%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 748 Da
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68431 (Residues: 2-7; Coverage: 4%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Polycomb protein SUZ12 Chains: L, T
Histone-binding protein RBBP4 Chain: N
Histone-lysine N-methyltransferase EZH2 Chain: C
Protein Jumonji Chain: F
Zinc finger protein AEBP2 Chain: P
Histone H3.1t Chain: I

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-43357
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli