› 8vo6

Electron Microscopy
3.1Å resolution

Cryo-EM structure of fascin bound to F-actin (actin binding site 2)

Released:
DOI: 10.2210/pdb8vo6/pdb
PDB entry 8vo6 coloured by chain, front view.
PDB entry 8vo6 coloured by chain, side view.
PDB entry 8vo6 coloured by chain, top view.
Source organisms:
Primary publication:
Fascin structural plasticity mediates flexible actin bundle construction.
Gong R, Reynolds MJ, Carney KR, Hamilton K, Bidone TC, Alushin GM
Nat Struct Mol Biol (2025)
PMID: 39833469
Related structures: EMD-43365

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-283929 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fascin Chain: A
Molecule details ›
Chain: A
Length: 498 amino acids
Theoretical weight: 55.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16658 (Residues: 1-493; Coverage: 100%)
Gene names: FAN1, FSCN1, HSN, SNL
Sequence domains: Fascin domain
Actin, alpha skeletal muscle Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 373 amino acids
Theoretical weight: 41.63 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P68139 (Residues: 5-377; Coverage: 99%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

2 bound ligands:
Ligand ADP 3 x ADP
Ligand MG 3 x MG
1 modified residue:
Ligand HIC 3 x HIC

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-43365
Expression system: Escherichia coli

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