› 8vrk

Electron Microscopy
8.5Å resolution

Rigid body fitted model for refined density map of gamma tubulin ring complex capped microtubule

Released:
DOI: 10.2210/pdb8vrk/pdb
PDB entry 8vrk coloured by chain, front view.
PDB entry 8vrk coloured by chain, side view.
PDB entry 8vrk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the γ-tubulin ring complex-capped microtubule.
Aher A, Urnavicius L, Xue A, Neselu K, Kapoor TM
Nat Struct Mol Biol (2024)
PMID: 38609661
Related structures: EMD-43483

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 60-mer (preferred)
PDBe Complex ID:
PDB-CPX-279866 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Tubulin alpha-1B chain Chains: 1, O, P, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: 1, O, P, Q, R, S, T, U, V, W, X, Y, Z
Length: 457 amino acids
Theoretical weight: 51.02 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P68363 (Residues: 1-42, 43-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta-3 chain Chains: 2, o, p, q, r, s, t, u, v, w, x, y, z
Molecule details ›
Chains: 2, o, p, q, r, s, t, u, v, w, x, y, z
Length: 456 amino acids
Theoretical weight: 51.28 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q13509 (Residues: 1-450; Coverage: 100%)
Gene names: TUBB3, TUBB4
Sequence domains:
Gamma-tubulin complex component 6 Chains: 3, 6, L
Gamma-tubulin complex component 3 Chains: 5, B, D, F, H, N
Mitotic-spindle organizing protein 1 Chains: 7, 8
Actin, cytoplasmic 1 Chain: 9
Gamma-tubulin complex component 2 Chains: A, C, E, G, M
Gamma-tubulin complex component 4 Chains: I, K
Gamma-tubulin complex component 5 Chain: J
Tubulin gamma-1 chain Chains: a, b, c, d, e, f, g, h, i, j, k, l, m, n

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 8.5Å
Relevant EMDB volumes: EMD-43483
Expression system: Trichoplusia ni

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Citations

3 citation in other articles

Structure of the native γ-tubulin ring complex capping spindle microtubules.
Dendooven et al. (2024)
2 more