› 8vsl

X-ray diffraction
1.42Å resolution

Binary structure of 14-3-3 sigma and ARAF phosphopeptide (pS214)

Released:
DOI: 10.2210/pdb8vsl/pdb
Model geometry
Fit model/data
PDB entry 8vsl coloured by chain, front view.
PDB entry 8vsl coloured by chain, side view.
PDB entry 8vsl coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Vickery HR, Virta JM, Pennings M, Konstantinidou M, van den Oetelaar M, Neitz RJ, Ottmann C, Brunsveld L, Arkin MR

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-290602 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Serine/threonine-protein kinase A-Raf Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.48 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10398 (Residues: 209-220; Coverage: 2%)
Gene names: ARAF, ARAF1, PKS, PKS2

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand MG 3 x MG
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2221
Unit cell:
a: 82.518Å b: 112.806Å c: 62.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.208
Expression systems:
  • Escherichia coli
  • Not provided

Quick links