› 8vt9

Electron Microscopy
2.9Å resolution

WT SthK in the presence of PIP2 and cAMP

Released:
DOI: 10.2210/pdb8vt9/pdb
PDB entry 8vt9 coloured by chain, front view.
PDB entry 8vt9 coloured by chain, side view.
PDB entry 8vt9 coloured by chain, top view.
Source organism: Spirochaeta thermophila
Primary publication:
PIP2 inhibits pore opening of the cyclic nucleotide-gated channel SthK.
Thon O, Wang Z, Schmidpeter PAM, Nimigean CM
Nat Commun 15 8230 (2024)
PMID: 39300080
Related structures: EMD-43520

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-binding domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 456 amino acids
Theoretical weight: 51.12 KDa
Source organism: Spirochaeta thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0GA88 (Residues: 1-420; Coverage: 98%)
Gene name: Spith_0644
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CMP 4 x CMP
Ligand PT5 4 x PT5
Ligand PCW 28 x PCW
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-43520
Expression system: Escherichia coli BL21(DE3)

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