› 8vuc

X-ray diffraction
2.5Å resolution

Structure of FabS1CE2-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor

Released:
DOI: 10.2210/pdb8vuc/pdb
PDB entry 8vuc coloured by chain, front view.
PDB entry 8vuc coloured by chain, side view.
PDB entry 8vuc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Antigen-binding fragments with improved crystal lattice packing and enhanced conformational flexibility at the elbow region as crystallization chaperones.
Bruce HA, Singer AU, Blazer LL, Luu K, Ploder L, Pavlenco A, Kurinov I, Adams JJ, Sidhu SS
OpenAccess logo Protein Sci 33 e5081 (2024)
PMID: 38924648

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-247038 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
S1CE2 VARIANT OF FAB-EPR-1 heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 24.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
S1CE3 VARIANT OF FAB-EPR-1 light chain Chains: C, G
Molecule details ›
Chains: C, G
Length: 212 amino acids
Theoretical weight: 23.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

3 bound ligands:
Ligand EDO 2 x EDO
Ligand SO4 3 x SO4
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P32
Unit cell:
a: 67.389Å b: 67.389Å c: 168.232Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.204 0.265
Expression system: Homo sapiens

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