› 8vui

X-ray diffraction
2.1Å resolution

Structure of FabS1CE-EPR-1, an elbow-locked Fab, in complex with the erythropoeitin receptor

Released:
DOI: 10.2210/pdb8vui/pdb
PDB entry 8vui coloured by chain, front view.
PDB entry 8vui coloured by chain, side view.
PDB entry 8vui coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Antigen-binding fragments with improved crystal lattice packing and enhanced conformational flexibility at the elbow region as crystallization chaperones.
Bruce HA, Singer AU, Blazer LL, Luu K, Ploder L, Pavlenco A, Kurinov I, Adams JJ, Sidhu SS
OpenAccess logo Protein Sci 33 e5081 (2024)
PMID: 38924648

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273296 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
S1CE VARIANT OF FAB-EPR-1 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 24.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
S1CE VARIANT OF FAB-EPR-1 light chain Chain: G
Molecule details ›
Chain: G
Length: 212 amino acids
Theoretical weight: 23.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Erythropoietin receptor Chain: D
Molecule details ›
Chain: D
Length: 232 amino acids
Theoretical weight: 25.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P19235 (Residues: 25-250; Coverage: 47%)
Gene name: EPOR
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand EDO 3 x EDO
Ligand NA 4 x NA
Ligand CL 5 x CL
Ligand NAG 1 x NAG
Ligand CIT 1 x CIT
Ligand NH4 1 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 56.39Å b: 71.93Å c: 197.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.24
Expression system: Homo sapiens

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