› 8vxa

Electron Microscopy
2.79Å resolution

Structure of HamB-DNA complex, conformation 1, from the Escherichia coli Hachiman defense system

Released:
DOI: 10.2210/pdb8vxa/pdb
PDB entry 8vxa coloured by chain, front view.
PDB entry 8vxa coloured by chain, side view.
PDB entry 8vxa coloured by chain, top view.
Source organisms:
Primary publication:
Hachiman is a genome integrity sensor.
Tuck OT, Adler BA, Armbruster EG, Lahiri A, Hu JJ, Zhou J, Pogliano J, Doudna JA
bioRxiv (2024)
PMID: 38464307
Related structures: EMD-43615

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241083 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DEAD/DEAH box helicase Chain: A
Molecule details ›
Chain: A
Length: 1174 amino acids
Theoretical weight: 130.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A426EXV0 (Residues: 1-1174; Coverage: 100%)
Gene name: DU321_04165
Sequence domains:
DNA (40-MER) Chains: B, C
Molecule details ›
Chains: B, C
Length: 40 nucleotides
Theoretical weight: 12.15 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.79Å
Relevant EMDB volumes: EMD-43615
Expression systems:
  • Escherichia coli BL21
  • Not provided

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