› 8vxy

Electron Microscopy
3.19Å resolution

Structure of HamA(E138A,K140A)B-plasmid DNA complex from the Escherichia coli Hachiman defense system

Released:
DOI: 10.2210/pdb8vxy/pdb
PDB entry 8vxy coloured by chain, front view.
PDB entry 8vxy coloured by chain, side view.
PDB entry 8vxy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Hachiman is a genome integrity sensor.
Tuck OT, Adler BA, Armbruster EG, Lahiri A, Hu JJ, Zhou J, Pogliano J, Doudna JA
bioRxiv (2024)
PMID: 38464307
Related structures: EMD-43643

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241085 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Anti-bacteriophage protein A/HamA C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 28.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6XGE5 (Residues: 1-259; Coverage: 100%)
Gene names: DU321_04160, HIA71_14550
Sequence domains: HamA
Helicase ATP-binding domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 1174 amino acids
Theoretical weight: 130.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A426EXV0 (Residues: 1-1174; Coverage: 100%)
Gene names: DU321_04165, HIA71_14545
Sequence domains:
Plasmid DNA Chain: C
Molecule details ›
Chain: C
Length: 31 nucleotides
Theoretical weight: 9.39 KDa
Plasmid DNA Chain: D
Molecule details ›
Chain: D
Length: 31 nucleotides
Theoretical weight: 9.66 KDa

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.19Å
Relevant EMDB volumes: EMD-43643
Expression system: Escherichia coli BL21

Quick links