› 8vy9

Electron Microscopy
2.88Å resolution

CryoEM structure of Ggust-coupled TAS2R14 with cholesterol and an intracellular tastant

Released:
DOI: 10.2210/pdb8vy9/pdb
PDB entry 8vy9 coloured by chain, front view.
PDB entry 8vy9 coloured by chain, side view.
PDB entry 8vy9 coloured by chain, top view.
Source organisms:
Primary publication:
Bitter taste receptor activation by cholesterol and an intracellular tastant.
Kim Y, Gumpper RH, Liu Y, Kocak DD, Xiong Y, Cao C, Deng Z, Krumm BE, Jain MK, Zhang S, Jin J, Roth BL
Nature (2024)
PMID: 38600377
Related structures: EMD-43650

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-242603 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(t) subunit alpha-3 Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 40.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A8MTJ3 (Residues: 1-52, 53-354; Coverage: 100%)
Gene name: GNAT3
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 402 amino acids
Theoretical weight: 42.94 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: D
Molecule details ›
Chain: D
Length: 267 amino acids
Theoretical weight: 28.67 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Taste receptor type 2 member 14 Chain: R
Molecule details ›
Chain: R
Length: 653 amino acids
Theoretical weight: 72.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NYV8 (Residues: 1-317; Coverage: 100%)
Gene name: TAS2R14
Sequence domains: Taste receptor protein (TAS2R)

Ligands and Environments

2 bound ligands:
Ligand A1AEI 1 x A1AEI
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-43650
Expression system: Spodoptera frugiperda

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