› 8vym

Electron Microscopy
3.4Å resolution

Soluble ectodomain of human cytomegalovirus (HCMV) glycoprotein B (gB) in the postfusion conformation in complex with 1G2 and 7H3 Fabs

Released:
DOI: 10.2210/pdb8vym/pdb
PDB entry 8vym coloured by chain, front view.
PDB entry 8vym coloured by chain, side view.
PDB entry 8vym coloured by chain, top view.
Source organisms:
Primary publication:
Structure-based design of a soluble human cytomegalovirus glycoprotein B antigen stabilized in a prefusion-like conformation.
Sponholtz MR, Byrne PO, Lee AG, Ramamohan AR, Goldsmith JA, McCool RS, Zhou L, Johnson NV, Hsieh CL, Connors M, Karthigeyan KP, Crooks CM, Fuller AS, Campbell JD, Permar SR, Maynard JA, Yu D, Bottomley MJ, McLellan JS
Proc Natl Acad Sci U S A 121 e2404250121 (2024)
PMID: 39231203
Related structures: EMD-43667

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279792 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (7 distinct):
Envelope glycoprotein B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 786 amino acids
Theoretical weight: 88.93 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: P13201 (Residues: 1-704; Coverage: 77%)
Gene names: UL55, gB
Sequence domains:
1G2 Fab Heavy Chain Chains: D, G, J
Molecule details ›
Chains: D, G, J
Length: 224 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
1G2 Fab Light Chain Chains: E, F, I
Molecule details ›
Chains: E, F, I
Length: 214 amino acids
Theoretical weight: 22.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
7H3 Fab Light Chain Chain: H
Molecule details ›
Chain: H
Length: 216 amino acids
Theoretical weight: 22.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
7H3 Fab Heavy Chain Chain: K
Molecule details ›
Chain: K
Length: 234 amino acids
Theoretical weight: 25.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 10 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-43667
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Beta Spike-Presenting SARS-CoV-2 Virus-like Particle Vaccine Confers Broad Protection against Other VOCs in Mice.
Ullah et al. (2024)