› 8vz4

X-ray diffraction
1.25Å resolution

L54G Mutant of E. coli Dihydrofolate Reductase Complexed with Nicotinamide Adenine Dinucleotide Phosphate (oxidized form)

Released:
DOI: 10.2210/pdb8vz4/pdb
Model geometry
Fit model/data
PDB entry 8vz4 coloured by chain, front view.
PDB entry 8vz4 coloured by chain, side view.
PDB entry 8vz4 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Fried SDE, Boxer SG

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 17.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABQ4 (Residues: 1-159; Coverage: 100%)
Gene names: JW0047, b0048, folA, tmrA
Sequence domains: Dihydrofolate reductase

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: P21
Unit cell:
a: 38.493Å b: 58.592Å c: 70.208Å
α: 90° β: 102.08° γ: 90°
R-values:
R R work R free
0.165 0.164 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO