› 8vzn

Electron Microscopy
2.77Å resolution

Cryo-EM structure of FLVCR2 in the inward-facing state with choline bound

Released:
DOI: 10.2210/pdb8vzn/pdb
PDB entry 8vzn coloured by chain, front view.
PDB entry 8vzn coloured by chain, side view.
PDB entry 8vzn coloured by chain, top view.
Source organisms:
Primary publication:
Structural and molecular basis of choline uptake into the brain by FLVCR2.
Cater RJ, Mukherjee D, Gil-Iturbe E, Erramilli SK, Chen T, Koo K, Santander N, Reckers A, Kloss B, Gawda T, Choy BC, Zhang Z, Katewa A, Larpthaveesarp A, Huang EJ, Mooney SWJ, Clarke OB, Yee SW, Giacomini KM, Kossiakoff AA, Quick M, Arnold T, Mancia F
Nature (2024)
PMID: 38693257
Related structures: EMD-43683

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275236 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Choline/ethanolamine transporter FLVCR2 Chain: A
Molecule details ›
Chain: A
Length: 551 amino acids
Theoretical weight: 60.1 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q91X85 (Residues: 1-551; Coverage: 100%)
Gene names: Flvcr2, Mfsd7c
Sequence domains: Major Facilitator Superfamily
Fab FLV9 heavy chain Chain: B
Molecule details ›
Chain: B
Length: 239 amino acids
Theoretical weight: 25.77 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Fab FLV9 light chain Chain: C
Molecule details ›
Chain: C
Length: 215 amino acids
Theoretical weight: 23.29 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CHT 1 x CHT
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.77Å
Relevant EMDB volumes: EMD-43683
Expression systems:
  • Homo sapiens
  • Escherichia coli

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