› 8w0a

Electron Microscopy
3.2Å resolution

Human DNA polymerase theta helicase domain in complex with ssDNA, dimer form

Released:
DOI: 10.2210/pdb8w0a/pdb
PDB entry 8w0a coloured by chain, front view.
PDB entry 8w0a coloured by chain, side view.
PDB entry 8w0a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Polθ-Helicase DNA Binding and Microhomology-Mediated End-Joining.
Ito F, Li Z, Minakhin L, Khant HA, Pomerantz RT, Chen XS
bioRxiv (2024)
PMID: 38895274
Related structures: EMD-43706

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270951 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase theta Chains: A, C
Molecule details ›
Chains: A, C
Length: 894 amino acids
Theoretical weight: 99.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75417 (Residues: 1-894; Coverage: 35%)
Gene names: POLH, POLQ
Sequence domains:
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*T)-3') Chains: B, D
Molecule details ›
Chains: B, D
Length: 45 nucleotides
Theoretical weight: 13.48 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-43706
Expression systems:
  • Escherichia coli
  • Not provided

Quick links