› 8w0f

Electron Microscopy
2.8Å resolution

Cryo-EM structure of a human MCM2-7 double hexamer on dsDNA

Released:
DOI: 10.2210/pdb8w0f/pdb
PDB entry 8w0f coloured by chain, front view.
PDB entry 8w0f coloured by chain, side view.
PDB entry 8w0f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multiple mechanisms for licensing human replication origins.
Yang R, Hunker O, Wise M, Bleichert F
Nature (2024)
PMID: 39604729
Related structures: EMD-43708

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-282720 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
DNA replication licensing factor MCM2 Chains: 2, A
Molecule details ›
Chains: 2, A
Length: 904 amino acids
Theoretical weight: 102.03 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49736 (Residues: 1-904; Coverage: 100%)
Gene names: BM28, CCNL1, CDCL1, KIAA0030, MCM2
Sequence domains:
DNA replication licensing factor MCM3 Chains: 3, B
Molecule details ›
Chains: 3, B
Length: 810 amino acids
Theoretical weight: 91.25 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25205 (Residues: 2-808; Coverage: 100%)
Gene name: MCM3
Sequence domains:
DNA replication licensing factor MCM4 Chains: 4, C
DNA replication licensing factor MCM5 Chains: 5, D
DNA replication licensing factor MCM6 Chains: 6, E
DNA replication licensing factor MCM7 Chains: 7, F
DNA (47-MER) Chain: O
DNA (47-MER) Chain: S

Ligands and Environments

4 bound ligands:
Ligand ZN 10 x ZN
Ligand MG 10 x MG
Ligand ATP 2 x ATP
Ligand ADP 8 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-43708
Expression system: Trichoplusia ni

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