› 8w1f

X-ray diffraction
3Å resolution

Crystal Structure of DPS-like protein PA4880 from Pseudomonas aeruginosa (dodecamer, Mg bound)

Released:
DOI: 10.2210/pdb8w1f/pdb
PDB entry 8w1f coloured by chain, front view.
PDB entry 8w1f coloured by chain, side view.
PDB entry 8w1f coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Pseudomonas aeruginosa gene PA4880 encodes a Dps-like protein with a Dps fold, bacterioferritin-type ferroxidase centers, and endonuclease activity.
Rajapaksha N, Yao H, Cook A, Seibold S, Liu L, Battaile KP, Fontenot L, Donnarumma F, Lovell S, Rivera M
Front Mol Biosci 11 1390745 (2024)
PMID: 38841187

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterioferritin Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 177 amino acids
Theoretical weight: 20.24 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HUT3 (Residues: 1-177; Coverage: 100%)
Gene name: PA4880
Sequence domains: Ferritin-like domain

Ligands and Environments

3 bound ligands:
Ligand MG 37 x MG
Ligand SO4 44 x SO4
Ligand FE2 24 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 215.79Å b: 215.79Å c: 277.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.204
Expression system: Escherichia coli BL21(DE3)

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