› 8w1o

Electron Microscopy
2.8Å resolution

Cryo-EM structure of BTV virion

Released:
DOI: 10.2210/pdb8w1o/pdb
PDB entry 8w1o coloured by chain, front view.
PDB entry 8w1o coloured by chain, side view.
PDB entry 8w1o coloured by chain, top view.
Source organism: Bluetongue virus (serotype 1 / isolate South Africa)
Primary publication:
RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells.
Xia X, Sung PY, Martynowycz MW, Gonen T, Roy P, Zhou ZH
Cell (2024)
PMID: 38614100
Related structures: EMD-43727

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-246231 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
Core protein VP3 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 901 amino acids
Theoretical weight: 103.41 KDa
Source organism: Bluetongue virus (serotype 1 / isolate South Africa)
UniProt:
  • Canonical: P56582 (Residues: 1-901; Coverage: 100%)
Gene names: L3, Segment-3
Sequence domains: Orbivirus VP3 (T2) protein
RNA-directed RNA polymerase Chain: K
Molecule details ›
Chain: K
Length: 1302 amino acids
Theoretical weight: 149.93 KDa
Source organism: Bluetongue virus (serotype 1 / isolate South Africa)
UniProt:
  • Canonical: W0G557 (Residues: 1-1302; Coverage: 100%)
Sequence domains: Orbivirus RNA-dependent RNA polymerase (VP1)
Outer capsid protein VP2 Chain: N
Molecule details ›
Chain: N
Length: 961 amino acids
Theoretical weight: 112.13 KDa
Source organism: Bluetongue virus (serotype 1 / isolate South Africa)
UniProt:
  • Canonical: B7U676 (Residues: 1-961; Coverage: 100%)
Gene name: L2
Sequence domains: Orbivirus outer capsid protein VP2
RNA-1 Chain: L
Molecule details ›
Chain: L
Length: 7 nucleotides
Theoretical weight: 2.17 KDa
RNA-2 Chain: M
Molecule details ›
Chain: M
Length: 9 nucleotides
Theoretical weight: 2.83 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-43727

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