› 8w2g

Electron Microscopy
3Å resolution

Human liver phosphofructokinase-1 in the R-state conformation

Released:
DOI: 10.2210/pdb8w2g/pdb
PDB entry 8w2g coloured by chain, front view.
PDB entry 8w2g coloured by chain, side view.
PDB entry 8w2g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for allosteric regulation of human phosphofructokinase-1.
Lynch EM, Hansen H, Salay L, Cooper M, Timr S, Kollman JM, Webb BA
Nat Commun 15 7323 (2024)
PMID: 39183237
Related structures: EMD-43747

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase, liver type Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 780 amino acids
Theoretical weight: 85.12 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P17858 (Residues: 1-780; Coverage: 100%)
Gene name: PFKL
Sequence domains: Phosphofructokinase

Ligands and Environments

3 bound ligands:
Ligand F6P 4 x F6P
Ligand ADP 12 x ADP
Ligand FBP 4 x FBP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-43747
Expression system: unidentified baculovirus

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