8w51

Electron Microscopy
2.4Å resolution

Structure of YchF(H114A) on E.coli 50S ribosomal subunit

Released:
Source organism: Escherichia coli
Entry authors: Yu T, Li X, Zeng F
Related structures: EMD-37271

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 32-mer (preferred)
PDBe Complex ID:
PDB-CPX-276005 (preferred)
Entry contents:
30 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (32 distinct):
Large ribosomal subunit protein uL2 Chain: C
Molecule details ›
Chain: C
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: D
Molecule details ›
Chain: D
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: E
Large ribosomal subunit protein uL5 Chain: F
Large ribosomal subunit protein uL6 Chain: G
Large ribosomal subunit protein bL9 Chain: H
Large ribosomal subunit protein uL11 Chain: J
Large ribosomal subunit protein uL13 Chain: K
Large ribosomal subunit protein uL14 Chain: L
Large ribosomal subunit protein uL15 Chain: M
Large ribosomal subunit protein uL16 Chain: N
Large ribosomal subunit protein bL17 Chain: O
Large ribosomal subunit protein uL18 Chain: P
Large ribosomal subunit protein bL19 Chain: Q
Large ribosomal subunit protein bL20 Chain: R
Large ribosomal subunit protein bL21 Chain: S
Large ribosomal subunit protein uL22 Chain: T
Large ribosomal subunit protein uL23 Chain: U
Large ribosomal subunit protein uL24 Chain: V
Large ribosomal subunit protein bL25 Chain: W
Large ribosomal subunit protein bL27 Chain: X
Large ribosomal subunit protein bL28 Chain: Y
Large ribosomal subunit protein uL29 Chain: Z
Large ribosomal subunit protein uL30 Chain: 0
Large ribosomal subunit protein bL32 Chain: 1
Large ribosomal subunit protein bL33 Chain: 2
Large ribosomal subunit protein bL34 Chain: 3
Large ribosomal subunit protein bL35 Chain: 4
Large ribosomal subunit protein bL36A Chain: 5
Ribosome-binding ATPase YchF Chain: y
23S ribosomal RNA Chain: A
5S ribosomal RNA Chain: B

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.4Å
Relevant EMDB volumes: EMD-37271
Expression system: Escherichia coli