› 8w5j

Electron Microscopy
4.4Å resolution

Cryo-EM structure of the yeast TOM core complex (from TOM-TIM23 complex)

Released:
DOI: 10.2210/pdb8w5j/pdb
PDB entry 8w5j coloured by chain, front view.
PDB entry 8w5j coloured by chain, side view.
PDB entry 8w5j coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The architecture of substrate-engaged TOM-TIM23 supercomplex reveals preprotein proximity sites for mitochondrial protein translocation.
Wang Q, Zhuang J, Huang R, Guan Z, Yan L, Hong S, Zhang L, Huang C, Liu Z, Yin P
OpenAccess logo Cell Discov 10 19 (2024)
PMID: 38360717
Related structures: EMD-37294

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150030 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Mitochondrial import receptor subunit TOM40 Chains: A, I
Molecule details ›
Chains: A, I
Length: 387 amino acids
Theoretical weight: 42.07 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23644 (Residues: 1-387; Coverage: 100%)
Gene names: ISP42, MOM38, TOM40, YM8325.04, YMR203W
Sequence domains: Eukaryotic porin
Mitochondrial import receptor subunit TOM22 Chains: B, J
Molecule details ›
Chains: B, J
Length: 152 amino acids
Theoretical weight: 16.8 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P49334 (Residues: 1-152; Coverage: 100%)
Gene names: MAS17, MAS22, MOM22, N1217, N1862, TOM22, YNL131W
Sequence domains: Mitochondrial import receptor subunit Tom22
Mitochondrial import receptor subunit TOM5 Chains: C, K
Molecule details ›
Chains: C, K
Length: 50 amino acids
Theoretical weight: 5.99 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P80967 (Residues: 1-50; Coverage: 100%)
Gene names: MOM8A, TOM5, YPR133W-A
Sequence domains: Mitochondrial import receptor subunit or translocase
Mitochondrial import receptor subunit TOM6 Chains: D, L
Molecule details ›
Chains: D, L
Length: 61 amino acids
Theoretical weight: 6.41 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33448 (Residues: 1-61; Coverage: 100%)
Gene names: ISP6, TOM6, YOR045W
Sequence domains: Mitochondrial import receptor subunit Tom6, fungal
Mitochondrial import receptor subunit TOM7 Chains: E, M
Molecule details ›
Chains: E, M
Length: 60 amino acids
Theoretical weight: 6.88 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53507 (Residues: 1-60; Coverage: 100%)
Gene names: MOM7, N2378, TOM7, YNL070W
Sequence domains: TOM7 family

Ligands and Environments

1 bound ligand:
Ligand 46E 1 x 46E
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-37294
Expression system: Saccharomyces cerevisiae S288C

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