› 8w5z

X-ray diffraction
1.55Å resolution

Crystal structure of tick tyrosylprotein sulfotransferase reveals the activation mechanism of tick anticoagulant protein madanin

Released:
DOI: 10.2210/pdb8w5z/pdb
PDB entry 8w5z coloured by chain, front view.
PDB entry 8w5z coloured by chain, side view.
PDB entry 8w5z coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of tick tyrosylprotein sulfotransferase reveals the activation mechanism of the tick anticoagulant protein madanin.
Yoshimura M, Teramoto T, Asano H, Iwamoto Y, Kondo M, Nishimoto E, Kakuta Y
J Biol Chem 300 105748 (2024)
PMID: 38354785

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270023 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein-tyrosine sulfotransferase Chains: A, C
Molecule details ›
Chains: A, C
Length: 393 amino acids
Theoretical weight: 44.8 KDa
Source organism: Ixodes
Expression system: Escherichia coli
UniProt:
  • Canonical: B7Q097 (Residues: 2-148, 149-328; Coverage: 100%)
Gene name: IscW_ISCW009297
Sequence domains: Sulfotransferase family
Madanin Y54-peptide Chains: B, D
Molecule details ›
Chains: B, D
Length: 11 amino acids
Theoretical weight: 1.32 KDa
Source organism: longicornis species complex
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand A3P 2 x A3P
Ligand 1PS 4 x 1PS
Ligand TRS 4 x TRS
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: C2221
Unit cell:
a: 147.13Å b: 151.19Å c: 93.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.195
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 mention without citation

Crystal structure of tick tyrosylprotein sulfotransferase reveals the activation mechanism of the tick anticoagulant protein madanin.
Yoshimura et al. (2024)