› 8w7j

Electron Microscopy
3.98Å resolution

Cryo-EM structure of ClassIII Lanthipeptide modification enzyme PneKC with chain A bounded to substrate PneA and GTP.

Released:
DOI: 10.2210/pdb8w7j/pdb
PDB entry 8w7j coloured by chain, front view.
PDB entry 8w7j coloured by chain, side view.
PDB entry 8w7j coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Mechanistic insights into lanthipeptide modification by a distinct subclass of LanKC enzyme that forms dimers.
Li Y, Shao K, Li Z, Zhu K, Gan BK, Shi J, Xiao Y, Luo M
Nat Commun 15 7090 (2024)
PMID: 39154050
Related structures: EMD-37339

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275632 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein kinase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 867 amino acids
Theoretical weight: 100.33 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
PneA LP Chain: C
Molecule details ›
Chain: C
Length: 14 amino acids
Theoretical weight: 1.21 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand GTP 1 x GTP
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.98Å
Relevant EMDB volumes: EMD-37339
Expression system: Escherichia coli

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