› 8w8d

Electron Microscopy
2.8Å resolution

Structural mechanism of inhibition of the Rho transcription termination factor by Rof

Released:
DOI: 10.2210/pdb8w8d/pdb
PDB entry 8w8d coloured by chain, front view.
PDB entry 8w8d coloured by chain, side view.
PDB entry 8w8d coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A widely conserved protein Rof inhibits transcription termination factor Rho and promotes Salmonella virulence program.
Zhang J, Zhang S, Zhou W, Zhang X, Li G, Li R, Lin X, Chen Z, Liu F, Shen P, Zhou X, Gao Y, Chen Z, Chao Y, Wang C
OpenAccess logo Nat Commun 15 3187 (2024)
PMID: 38622116
Related structures: EMD-37342

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-272659 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription termination factor Rho Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 419 amino acids
Theoretical weight: 47.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG30 (Residues: 1-419; Coverage: 100%)
Gene names: JW3756, b3783, nitA, psuA, rho, rnsC, sbaA, tsu
Sequence domains:
Protein rof Chains: a, b, c, d, e, f
Molecule details ›
Chains: a, b, c, d, e, f
Length: 84 amino acids
Theoretical weight: 9.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFW8 (Residues: 1-84; Coverage: 100%)
Gene names: JW0184, b0189, rof, yaeO
Sequence domains: Modulator of Rho-dependent transcription termination (ROF)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-37342
Expression system: Escherichia coli

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