› 8w8j

X-ray diffraction
1.81Å resolution

Crystal structure of bacterial prolyl-tRNA synthetase in complex with inhibitor PAA-19

Released:
DOI: 10.2210/pdb8w8j/pdb
PDB entry 8w8j coloured by chain, front view.
PDB entry 8w8j coloured by chain, side view.
PDB entry 8w8j coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Luo Z, Zhou H

Function and Biology Details

Reaction catalysed: 
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proline--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 579 amino acids
Theoretical weight: 64.19 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I502 (Residues: 1-571; Coverage: 100%)
Gene names: PA0956, proS
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand W1Q 2 x W1Q
Ligand EDO 17 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 74.668Å b: 102.38Å c: 193.477Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.208
Expression system: Escherichia coli

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