› 8w8w

Electron Microscopy
2.9Å resolution

Cryo-EM structure of alpha-MSH-MC3R-Gs_Nb35 complex

Released:
DOI: 10.2210/pdb8w8w/pdb
PDB entry 8w8w coloured by chain, front view.
PDB entry 8w8w coloured by chain, side view.
PDB entry 8w8w coloured by chain, top view.
Source organisms:
Entry authors: Chen Q, Fu J
Related structures: EMD-37359

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-290588 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Melanocortin receptor 3; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 602 amino acids
Theoretical weight: 67.05 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: P41968 (Residues: 2-323; Coverage: 100%)
Gene names: MC3R, cybC
Sequence domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 384 amino acids
Theoretical weight: 41.73 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Nanobody35 Chain: N
alpha-MSH Chain: L
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.9Å
Relevant EMDB volumes: EMD-37359
Expression systems:
  • Spodoptera frugiperda
  • Eschenbachia
  • Not provided

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