› 8w9u

X-ray diffraction
1.85Å resolution

Crystal structure of CspR from Bacillus subtilis complexed with SAH

Released:
DOI: 10.2210/pdb8w9u/pdb
PDB entry 8w9u coloured by chain, front view.
PDB entry 8w9u coloured by chain, side view.
PDB entry 8w9u coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Entry authors: Lee Y, Kim J

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-519383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative tRNA (cytidine(34)-2'-O)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.77 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31590 (Residues: 2-160; Coverage: 99%)
Gene names: BSU08930, cspR, ygaR
Sequence domains: SpoU rRNA Methylase family

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P1
Unit cell:
a: 39.763Å b: 46.959Å c: 50.09Å
α: 110.33° β: 92.71° γ: 106.56°
R-values:
R R work R free
0.197 0.195 0.238
Expression system: Escherichia coli BL21(DE3)

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