› 8wa5

Electron Microscopy
2.51Å resolution

Cryo-EM structure of the gastric proton pump Y799W/E936Q mutant in K+-occluded (K+)E2-AlF state

Released:
DOI: 10.2210/pdb8wa5/pdb
PDB entry 8wa5 coloured by chain, front view.
PDB entry 8wa5 coloured by chain, side view.
PDB entry 8wa5 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Specific protonation of acidic residues confers K+ selectivity to the gastric proton pump.
Madapally HV, Abe K, Dubey V, Khandelia H
J Biol Chem 300 105542 (2023)
PMID: 38072058
Related structures: EMD-37391

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + H(+)(Side 1) + K(+)(Side 2) = ADP + phosphate + H(+)(Side 2) + K(+)(Side 1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148371 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Potassium-transporting ATPase alpha chain 1 Chain: A
Molecule details ›
Chain: A
Length: 1033 amino acids
Theoretical weight: 114.28 KDa
Source organism: Sus scrofa
Expression system: Homo sapiens
UniProt:
  • Canonical: P19156 (Residues: 2-1034; Coverage: 100%)
Gene name: ATP4A
Sequence domains:
Potassium-transporting ATPase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 290 amino acids
Theoretical weight: 33.11 KDa
Source organism: Sus scrofa
Expression system: Homo sapiens
UniProt:
  • Canonical: P18434 (Residues: 1-290; Coverage: 100%)
Gene name: ATP4B
Sequence domains: Sodium / potassium ATPase beta chain

Ligands and Environments

6 bound ligands:
Ligand K 3 x K
Ligand ALF 1 x ALF
Ligand MG 1 x MG
Ligand CLR 1 x CLR
Ligand PCW 1 x PCW
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.51Å
Relevant EMDB volumes: EMD-37391
Expression system: Homo sapiens

Quick links

Citations

1 mention without citation

Specific protonation of acidic residues confers K<sup>+</sup> selectivity to the gastric proton pump.
Madapally et al. (2024)