› 8wb0

Electron Microscopy
2.94Å resolution

De novo transcribing complex 17 (TC17), the early elongation complex with Pol II positioned 17nt downstream of TSS

Released:
DOI: 10.2210/pdb8wb0/pdb
PDB entry 8wb0 coloured by chain, front view.
PDB entry 8wb0 coloured by chain, side view.
PDB entry 8wb0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural visualization of transcription initiation in action.
Chen X, Liu W, Wang Q, Wang X, Ren Y, Qu X, Li W, Xu Y
Science 382 eadi5120 (2023)
PMID: 38127763
Related structures: EMD-37411

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-286384 (preferred)
Entry contents:
15 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (18 distinct):
Alpha-amanitin Chain: N
Molecule details ›
Chain: N
Length: 8 amino acids
Theoretical weight: 939 Da
Source organism: Amanita phalloides
UniProt:
  • Canonical: P85421 (Residues: 1-8; Coverage: 35%)
General transcription factor IIF subunit 1 Chain: S
Molecule details ›
Chain: S
Length: 517 amino acids
Theoretical weight: 58.34 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P35269 (Residues: 1-517; Coverage: 100%)
Gene names: GTF2F1, RAP74
Sequence domains: Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
General transcription factor IIF subunit 2 Chain: T
DNA-directed RNA polymerase subunit Chain: o
DNA-directed RNA polymerase subunit beta Chain: p
DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein Chain: q
RNA polymerase Rpb4/RPC9 core domain-containing protein Chain: r
DNA-directed RNA polymerase II subunit E Chain: s
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: t
DNA-directed RNA polymerase subunit Chain: u
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: v
DNA-directed RNA polymerase II subunit RPB9 Chain: w
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: x
DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein Chain: y
RNA polymerase II, I and III subunit K Chain: z
non-template DNA Chain: X
template DNA Chain: Y
RNA Chain: Z

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand MG 1 x MG
Ligand W0F 1 x W0F
5 modified residues:
Ligand ILX 1 x ILX
Ligand TRX 1 x TRX
Ligand CSX 1 x CSX
Ligand HYP 1 x HYP
Ligand G2L 1 x G2L

Experiments and Validation Details

Entry percentile scores
Resolution: 2.94Å
Relevant EMDB volumes: EMD-37411
Expression system: Homo sapiens

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